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Hair Follicle Drug Test: How It Works | AMH Nationwide

Hair Follicle Drug Test How It Works for Reliable Substance Detection

Hair follicle drug test how it works starts with collecting a small hair sample, which is then analyzed by national labs using screening and confirmation tests. Every non-negative result undergoes review by a certified Medical Review Officer. Call (877) 731-6362 for more information.

COLLECTION

How is a hair follicle drug test sample collected?

A hair follicle drug test sample is collected by cutting a small amount of hair close to the scalp, usually from the crown or back of the head. The sample typically measures about 1.5 inches in length and weighs approximately 100 milligrams.

The collection is performed at a designated site directed by AMH Nationwide. The process is non-invasive and usually takes only a few minutes. If scalp hair is unavailable, body hair may be collected as an alternative.

Sample size: about 100 mg of hair, 1.5 inches long
Collection site: crown or back of the head
Alternative: body hair if scalp hair is insufficient
Collection supervised by trained personnel at a national lab network site
LAB ANALYSIS

What happens to the hair sample at the lab?

Once collected, the hair sample is sent to one of five national lab networks: Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, or ExperTox. These labs follow strict protocols to ensure accurate analysis.

The lab first performs an initial immunoassay screening to detect the presence of drugs or their metabolites embedded in the hair shaft. This screening identifies potential positive samples that require further testing.

Labs involved: Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, ExperTox
First step: immunoassay screening for drug presence
Screening detects drugs incorporated into hair from bloodstream exposure
CONFIRMATION

How are positive hair follicle drug test results confirmed?

Samples that screen positive undergo confirmatory testing using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS). These methods precisely identify and quantify specific drug compounds.

Confirmation testing reduces the risk of false positives and verifies the initial screening results. Only confirmed positives are reported as positives.

Methods: GC-MS or LC-MS/MS for confirmation
Purpose: precise identification and quantification of drugs
Ensures reliability by eliminating false positives
MRO REVIEW

What role does the Medical Review Officer play in the process?

Every non-negative hair follicle drug test result is reviewed by a certified Medical Review Officer (MRO). The MRO evaluates the test findings in the context of medical history, prescription medications, and other relevant information.

The MRO may contact the individual to verify legitimate medical explanations before finalizing the test result. This step ensures accuracy and fairness in reporting.

MRO reviews all non-negative results
MRO verifies medical explanations for positive findings
Adds an expert clinical layer to the testing process

Need a Hair Follicle Drug Test?

Call (877) 731-6362 to schedule a collection appointment or get detailed information about the hair follicle drug test process.

PROCESS FLOW

What is the complete step-by-step process for a hair follicle drug test?

The hair follicle drug test process begins with AMH Nationwide directing the requester to a collection site within the five national lab networks. After sample collection, the hair is analyzed through screening and confirmation phases before MRO review.

This systematic approach ensures comprehensive detection and reliable results.

StepDescription
1. CollectionHair sample cut close to scalp at authorized site
2. ScreeningInitial immunoassay test for drug presence
3. ConfirmationPositive screens tested by GC-MS or LC-MS/MS
4. MRO ReviewCertified Medical Review Officer evaluates non-negative results
5. ReportingFinal test results sent to the requester
TESTING LIMITATIONS

What are the detection windows and limitations of hair follicle drug testing?

Hair follicle drug tests typically detect drug use within the previous 90 days, as drugs incorporate into hair as it grows. This makes it effective for identifying longer-term usage rather than recent use.

However, hair color, cosmetic treatments, and individual metabolism can affect detection sensitivity. Hair testing does not detect very recent drug intake within the past 5-7 days.

Detection window: approximately 90 days
Not suitable for detecting very recent use (within 5-7 days)
Factors affecting results: hair color, chemical treatments, growth rate
AMH
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AMH Nationwide Customer Service

AMH Nationwide is BBB A+ accredited and routes every order through Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, or ExperTox, all of which use multiple lab certification agencies and standards such as SAMHSA-aligned laboratory protocols, the ANSI National Accreditation Board for forensic testing and calibration, the College of American Pathologists (CAP), and Clinical Laboratory Improvement Amendments (CLIA). Every non-negative screen is reviewed by a certified Medical Review Officer as part of standard processing before a result is released, and chain-of-custody documentation is maintained on every collection. This page does not constitute medical or legal advice.

Last updated: July 2026
Frequently Asked Questions

Common Questions

Yes, body hair can be collected if scalp hair is insufficient. However, body hair may have a less precise detection window due to differing growth rates.

Results typically take several days to a week, depending on lab workload and confirmation testing requirements.

No, hair follicle tests are not subject to DOT urine testing regulations under 49 CFR Part 40 and are used mainly for non-DOT roles.

Commonly detected substances include marijuana, cocaine, opiates, amphetamines, PCP, and others, depending on the test panel.

No, it is a non-invasive procedure involving cutting a small hair sample close to the scalp without pain.

A certified Medical Review Officer reviews all non-negative results to verify any legitimate medical explanations before final reporting.

Ready to get started?

AMH Nationwide provides the lab order and arranges collection nationwide. Every non-negative result reviewed by a certified Medical Review Officer. Call (877) 731-6362 or contact us.

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