--- title: "Syphilis Test: Which Test Do I Need | AMH Nationwide" description: "Learn which syphilis test fits your situation. This syphilis test which test guide helps employers, courts, and individuals. Call (877) 731-6362." author: "AMH Nationwide" date: "2026-08-27" tags: ["syphilis test which test"] canonical: "https://amhnationwide.com/syphilis-test-which-test/" last_updated: "2026-08-27" --- # Syphilis Test: Which Test Do I Need | AMH Nationwide **By AMH Nationwide** | August 27, 2026 Learn which syphilis test fits your situation. This syphilis test which test guide helps employers, courts, and individuals. Call (877) 731-6362. --- The syphilis test which test question depends on your reason for testing, such as employer requirements, court orders, probation, or personal health. Syphilis testing is routed through five national lab networks, ensuring reliable results. Call (877) 731-6362 to discuss the appropriate syphilis test for your situation. TEST BASICS ## What is a syphilis test and which test options are available? A syphilis test detects antibodies or the bacteria Treponema pallidum that cause syphilis infection. The main tests include nontreponemal tests (RPR and VDRL) and treponemal tests (FTA-ABS, TPPA, and EIA). Nontreponemal tests screen for active infection and are often used first. Treponemal tests confirm the diagnosis and detect past or treated infections. Nontreponemal tests: RPR (Rapid Plasma Reagin), VDRL (Venereal Disease Research Laboratory) Treponemal tests: FTA-ABS (Fluorescent Treponemal Antibody Absorption), TPPA (Treponema pallidum Particle Agglutination), EIA (Enzyme Immunoassay) EMPLOYER TESTING ## When is this syphilis test the right choice for employers? Employers may require syphilis testing for certain industries such as healthcare or public safety. The syphilis test which test needed usually starts with a nontreponemal screening test like RPR. If the screening is positive, a treponemal confirmatory test is required. AMH Nationwide directs employers to collection sites with access to all five national lab networks for accurate and certified testing. Use nontreponemal test (RPR) for initial employee screening Confirm positives with treponemal test (FTA-ABS or TPPA) No online ordering; employers must call (877) 731-6362 to arrange testing COURT & LEGAL ## Which syphilis test is appropriate for court or legal requirements? Courts and legal entities often require documented syphilis testing results for cases involving sexual offenses, custody, or divorce. The syphilis test which test used must be a laboratory-based serologic test with clear chain of custody. AMH Nationwide routes samples through [Quest Diagnostics](https://www.questdiagnostics.com/), [LabCorp](https://www.labcorp.com/), Omega Labs, United States Drug Testing Labs, or ExperTox, ensuring certified results. Typically, a nontreponemal test followed by a treponemal test is standard. Nontreponemal test (RPR or VDRL) for screening Treponemal test (FTA-ABS, TPPA) for confirmation Testing directed through certified labs with chain of custody protocols [PROBATION](https://amhnationwide.com/hair-follicle-drug-test-probation/) TESTING ## Is the syphilis test the right test for probation requirements? Probation conditions may include [STD testing](https://amhnationwide.com/std-testing/) when related to criminal charges or health monitoring. The syphilis test which test is appropriate depends on the probation officer’s instructions but generally follows the two-step testing approach. A nontreponemal test screens for active infection, and a treponemal test confirms the diagnosis. AMH Nationwide ensures all tests undergo Medical Review Officer evaluation for accuracy. Follow probation instructions for test type Nontreponemal test first, then confirm with treponemal test if needed Call (877) 731-6362 to schedule testing at a local collection site ## Need Help Choosing the Right Syphilis Test? Call (877) 731-6362 to speak with a representative who can guide you to the appropriate syphilis test based on your specific situation. [Call (877) 731-6362](tel:+18777316362)[Contact Us](https://amhnationwide.com/contact-us/) PERSONAL TESTING ## Which syphilis test should individuals choose for personal health? Individuals seeking syphilis testing for personal health reasons should start with a nontreponemal test to detect current infection. If positive, a treponemal test confirms exposure and guides treatment. AMH Nationwide does not offer online ordering. Individuals must call (877) 731-6362 to be directed to a nearby collection site linked to one of the five national lab networks. Nontreponemal test (RPR) for initial screening Treponemal test (EIA or FTA-ABS) for confirmation Results reviewed by a certified Medical Review Officer TEST PROCESS ## How does AMH Nationwide handle syphilis test collection and processing? AMH Nationwide provides the lab order and directs the requester to a collection site within five national lab networks. There is no online ordering; all appointments and instructions are arranged by phone. All non-negative screens are reviewed by a certified Medical Review Officer. This process ensures accuracy and compliance with regulatory standards. Sample collected at authorized collection site Specimen sent to Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, or ExperTox Medical Review Officer reviews all non-negative results AMH Reviewed & Maintained By AMH Nationwide Customer Service AMH Nationwide is BBB A+ accredited and routes every order through Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, or ExperTox, all of which use multiple lab certification agencies and standards such as [SAMHSA](https://www.samhsa.gov/workplace/drug-testing)-aligned laboratory protocols, the ANSI National Accreditation Board for forensic testing and calibration, the College of American Pathologists (CAP), and Clinical Laboratory Improvement Amendments (CLIA). Every non-negative screen is reviewed by a certified Medical Review Officer as part of standard processing before a result is released, and chain-of-custody documentation is maintained on every collection. This page does not constitute medical or legal advice. **Last updated:** July 2026 You May Also Need [Nail Drug TestingNo head or body hair available? A 5-panel fingernail test covers a similar look-back window.Learn more →](https://amhnationwide.com/5-panel-fingernail-drug-test/)[ChildGuard: Environmental ExposureConcerned about a child or loved one? ChildGuard screens for exposure to drugs in their environment.Learn more →](https://amhnationwide.com/5-panel-hair-drug-test-environmental-exposure-childguard/)[Shorten the Detection WindowShortened options like 30 and 60 day hair panels are also available, useful when a urine test’s 1-3 day window is too short.Learn more →](https://amhnationwide.com/30-day-hair-drug-test/)[Hair ETG Alcohol TestAlso need alcohol testing? The hair ETG test screens for alcohol use, and can be added to the same collection.Learn more →](https://amhnationwide.com/etg-and-peth/) Frequently Asked Questions ## Common Questions Nontreponemal tests detect antibodies indicating active infection and are used for screening. Treponemal tests detect antibodies specific to syphilis bacteria and confirm diagnosis or past infection. No, AMH Nationwide does not offer online ordering. To arrange testing, call (877) 731-6362 for lab order and collection site directions. A certified Medical Review Officer reviews every non-negative syphilis test result as part of standard processing to ensure accuracy. DOT-regulated testing requires federally certified [urine](https://amhnationwide.com/urine-drug-testing/) tests under [49 CFR Part 40](https://www.transportation.gov/odapc/part40), which do not include syphilis testing. Syphilis tests are typically blood-based and used for non-DOT roles. Turnaround times vary by lab and test panel. Contact AMH Nationwide at (877) 731-6362 for estimated timing based on your test. Treponemal tests detect antibodies from past or treated infections, while nontreponemal tests usually indicate current active infection. Sources • [SAMHSA – Mandatory Guidelines for Federal Workplace Drug Testing Programs](https://www.samhsa.gov/workplace/drug-testing) • [U.S. DOT – 49 CFR Part 40 – Procedures for Transportation Workplace Drug and Alcohol Testing Programs](https://www.transportation.gov/odapc/part40) • [CDC – National Center for Health Statistics – Drug Use FastStats](https://www.cdc.gov/nchs/fastats/drug-use.htm) • [Quest Diagnostics – Employer Solutions, Hair Testing Methodology](https://www.questdiagnostics.com/) • [LabCorp – Occupational Testing Services](https://www.labcorp.com/) ## Ready to get started? AMH Nationwide provides the lab order and arranges collection nationwide. Every non-negative result reviewed by a certified Medical Review Officer. Call (877) 731-6362 or contact us. [Call (877) 731-6362](tel:+18777316362)[Contact Us](https://amhnationwide.com/contact-us/) --- **Need a drug, alcohol, HIV or STD test?** AMH Nationwide is a national lab testing agency operating since 2004, with more than 20,000 collection sites across the United States. Hair, urine, fingernail and blood testing, panels from 5 through 19, for individuals and employers. - Call: (877) 731-6377 - Find a collection site: https://amhnationwide.com/locations/ - Full page: https://amhnationwide.com/syphilis-test-which-test/