--- title: "Does dot do hair follicle test" description: "Does DOT do hair follicle test for drug screening? DOT requires federally certified urine testing, not hair. Learn more. Call (877) 731-6362." author: "AMH Nationwide" date: "2026-08-28" tags: ["does dot do hair follicle test", "does dot do hair follicle test | does dot do hair follicle drug test"] canonical: "https://amhnationwide.com/does-dot-do-hair-follicle-test/" last_updated: "2026-08-28" --- # Does dot do hair follicle test **By AMH Nationwide** | August 28, 2026 Does DOT do hair follicle test for drug screening? DOT requires federally certified urine testing, not hair. Learn more. Call (877) 731-6362. --- Does DOT do hair follicle test? The Department of Transportation (DOT) does not use hair follicle testing for federally regulated safety-sensitive drug testing. Instead, DOT mandates urine testing following strict 49 CFR Part 40 protocols. Hair tests are only used for non-DOT roles. For detailed guidance and testing services, call (877) 731-6362. DOT Testing Overview ## Does DOT use hair follicle testing for drug screening? DOT does not use hair follicle drug testing for federally regulated safety-sensitive positions. The Department of Transportation requires [urine](https://amhnationwide.com/urine-drug-testing/) drug testing that follows the federally certified protocol under [49 CFR Part 40](https://www.transportation.gov/odapc/part40). Hair follicle testing is not approved for DOT drug testing programs and is therefore not used for compliance. DOT’s drug testing program covers roles such as commercial drivers, pilots, and other safety-sensitive employees in transportation industries. The drug testing methods and specimen types are strictly regulated to ensure consistency and reliability. DOT-approved specimen: Urine only Hair follicle testing: Not accepted for DOT Regulation: 49 CFR Part 40 governs DOT drug testing Purpose: Safety-sensitive employee screening Non-DOT employers may use hair follicle testing for [pre-employment](https://amhnationwide.com/pre-employment-hair-testing/) or other screening purposes, but DOT-regulated testing requires urine specimens collected under specific federal protocols. Why urine testing? ## Why does DOT require urine testing instead of hair follicle testing? Urine testing is required by DOT because it provides a reliable, federally certified method to detect recent drug use within a timeframe relevant to workplace safety. The urine drug test follows strict collection, chain of custody, and laboratory analysis procedures outlined in 49 CFR Part 40. Hair follicle testing detects drug use over a longer period, typically up to 90 days, but is not certified under DOT protocols. DOT prioritizes testing methods that detect recent impairment or use to protect safety-sensitive functions. Hair follicle test basics ## What is a hair follicle drug test and who uses it? A hair follicle drug test analyzes a small sample of hair to detect drug metabolites deposited over time. It can identify drug use typically within the previous 90 days. This test is often used by employers for pre-employment screening or by courts and [probation](https://amhnationwide.com/hair-follicle-drug-test-probation/) offices where a longer [detection window](https://amhnationwide.com/hair-follicle-drug-test-detection-window/) is desired. Unlike DOT testing, hair follicle tests are not regulated by federal transportation rules and do not follow the 49 CFR Part 40 urine testing protocol. Many non-DOT employers and agencies use hair testing for its extended detection period. Collection and labs ## How does AMH Nationwide handle DOT and hair follicle testing? AMH Nationwide coordinates DOT drug testing and hair follicle testing through five national lab networks: [Quest Diagnostics](https://www.questdiagnostics.com/), [LabCorp](https://www.labcorp.com/), Omega Labs, United States Drug Testing Labs, and ExperTox. We provide the lab order and direct the requester to a collection site but do not offer online ordering. For DOT-regulated tests, urine specimens are collected according to the federally certified protocol. Hair follicle tests are available for non-DOT testing needs. Every non-negative screen is reviewed by a certified Medical Review Officer as part of standard processing. ## Need DOT-Compliant Drug Testing? Contact AMH Nationwide to schedule federally certified urine drug testing or non-DOT hair follicle testing. Call (877) 731-6362 for assistance. [Call (877) 731-6362](tel:+18777316362)[Contact Us](https://amhnationwide.com/contact-us/) Detection windows ## What are the detection windows for urine versus hair follicle tests? Urine drug tests detect recent drug use generally within 1 to 4 days depending on the substance and usage pattern. This short window aligns with DOT’s safety focus. Hair follicle tests detect drug use over a much longer period, roughly up to 90 days prior to collection. The choice between urine and hair testing depends on the employer’s or agency’s requirements. DOT mandates urine testing for safety-sensitive positions, while hair testing serves in non-DOT contexts where longer history is relevant. Ordering and compliance ## Can I order a DOT hair follicle test through AMH Nationwide? No, you cannot order a DOT hair follicle test because DOT does not use hair testing for federally regulated drug screening. AMH Nationwide provides the lab order and directs you to a collection site for DOT urine testing or non-DOT hair follicle testing according to your needs. To arrange DOT urine testing or hair follicle testing for other purposes, contact AMH Nationwide by calling (877) 731-6362. Pricing varies by panel and detection window. We do not offer online ordering. AMH Reviewed & Maintained By AMH Nationwide Customer Service AMH Nationwide is BBB A+ accredited and routes every order through Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, or ExperTox, all of which use multiple lab certification agencies and standards such as [SAMHSA](https://www.samhsa.gov/workplace/drug-testing)-aligned laboratory protocols, the ANSI National Accreditation Board for forensic testing and calibration, the College of American Pathologists (CAP), and Clinical Laboratory Improvement Amendments (CLIA). Every non-negative screen is reviewed by a certified Medical Review Officer as part of standard processing before a result is released, and chain-of-custody documentation is maintained on every collection. This page does not constitute medical or legal advice. **Last updated:** July 2026 You May Also Need [Nail Drug TestingNo head or body hair available? A 5-panel fingernail test covers a similar look-back window.Learn more →](https://amhnationwide.com/5-panel-fingernail-drug-test/)[ChildGuard: Environmental ExposureConcerned about a child or loved one? ChildGuard screens for exposure to drugs in their environment.Learn more →](https://amhnationwide.com/5-panel-hair-drug-test-environmental-exposure-childguard/)[Shorten the Detection WindowShortened options like 30 and 60 day hair panels are also available, useful when a urine test’s 1-3 day window is too short.Learn more →](https://amhnationwide.com/30-day-hair-drug-test/)[Hair ETG Alcohol TestAlso need alcohol testing? The hair ETG test screens for alcohol use, and can be added to the same collection.Learn more →](https://amhnationwide.com/etg-and-peth/) Frequently Asked Questions ## Common Questions No, the DOT only accepts urine drug tests collected under the federally certified protocol outlined in 49 CFR Part 40 for safety-sensitive employees. Hair follicle testing is not federally certified for DOT drug testing and does not meet the required protocols focused on detecting recent drug use. Yes, hair follicle tests can detect drug use up to 90 days prior, whereas urine tests detect recent use generally within a few days. Employers outside DOT regulations, courts, and probation agencies often order hair follicle tests for longer detection periods. No, AMH Nationwide provides DOT urine drug testing and non-DOT hair follicle testing through national lab networks but does not offer DOT hair testing. Call AMH Nationwide at (877) 731-6362 to receive a lab order and be directed to a collection site for federally certified DOT urine drug testing. Sources • [SAMHSA – Mandatory Guidelines for Federal Workplace Drug Testing Programs](https://www.samhsa.gov/workplace/drug-testing) • [U.S. DOT – 49 CFR Part 40 – Procedures for Transportation Workplace Drug and Alcohol Testing Programs](https://www.transportation.gov/odapc/part40) • [CDC – National Center for Health Statistics – Drug Use FastStats](https://www.cdc.gov/nchs/fastats/drug-use.htm) • [Quest Diagnostics – Employer Solutions, Hair Testing Methodology](https://www.questdiagnostics.com/) • [LabCorp – Occupational Testing Services](https://www.labcorp.com/) ## Ready to get started? AMH Nationwide provides the lab order and arranges collection nationwide. Every non-negative result reviewed by a certified Medical Review Officer. Call (877) 731-6362 or contact us. [Call (877) 731-6362](tel:+18777316362)[Contact Us](https://amhnationwide.com/contact-us/) --- **Need a drug, alcohol, HIV or STD test?** AMH Nationwide is a national lab testing agency operating since 2004, with more than 20,000 collection sites across the United States. Hair, urine, fingernail and blood testing, panels from 5 through 19, for individuals and employers. - Call: (877) 731-6377 - Find a collection site: https://amhnationwide.com/locations/ - Full page: https://amhnationwide.com/does-dot-do-hair-follicle-test/