--- title: "5-Panel Hair Follicle Drug Test: How It Works | AMH Nationwide" description: "Learn how a 5-panel hair follicle drug test works from sample collection to lab analysis and MRO review. Call (877) 731-6362 for details." author: "AMH Nationwide" date: "2026-08-27" tags: ["5-panel hair follicle drug test how it works"] canonical: "https://amhnationwide.com/5-panel-hair-follicle-drug-test-how-it-works/" last_updated: "2026-08-27" --- # 5-Panel Hair Follicle Drug Test: How It Works | AMH Nationwide **By AMH Nationwide** | August 27, 2026 Learn how a 5-panel hair follicle drug test works from sample collection to lab analysis and MRO review. Call (877) 731-6362 for details. --- A 5-panel hair follicle drug test how it works involves collecting a small hair sample, which is then analyzed through a two-step lab process including screening and confirmation. Every non-negative result undergoes Medical Review Officer evaluation. This test detects drug use over several months. Call (877) 731-6362 to arrange testing. COLLECTION ## How is the hair sample collected in a 5-panel hair follicle drug test? The hair sample collection begins with cutting a small amount of hair close to the scalp, typically from the crown area. Approximately 1.5 inches of hair length is needed, representing about 90 days of drug history. The sample size is about 100 to 120 strands, enough for laboratory analysis. Collection is performed at an authorized site directed by AMH Nationwide. The technician ensures the sample is properly labeled and sealed to maintain chain of custody. The sample is then sent to one of five national lab networks: [Quest Diagnostics](https://www.questdiagnostics.com/), [LabCorp](https://www.labcorp.com/), Omega Labs, United States Drug Testing Labs, or ExperTox for processing. LAB PROCESS ## What happens to the hair sample once it reaches the lab? Once the lab receives the hair sample, it undergoes a two-stage testing process. The first stage is an immunoassay screening designed to detect the presence of specific drug metabolites. If the screening is negative, the test is reported as negative without further testing. If the screening is positive or inconclusive, the sample proceeds to confirmation testing using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS). These methods provide definitive identification and quantification of drugs in the hair sample. This two-step approach ensures high accuracy and reduces false positives. The panel includes five drug classes typically: marijuana (THC), cocaine, opiates, amphetamines, and phencyclidine (PCP). DRUGS DETECTED ## Which drugs are detected in a 5-panel hair follicle drug test? A 5-panel hair follicle drug test detects five common drug classes by analyzing metabolites embedded in the hair shaft. These include: – Marijuana (THC) – Cocaine – Opiates (such as morphine, codeine, heroin) – Amphetamines (including methamphetamine and MDMA) – Phencyclidine (PCP) The hair sample reflects drug use over a [detection window](https://amhnationwide.com/hair-follicle-drug-test-detection-window/) of approximately 90 days, depending on hair length. This makes the test suitable for identifying long-term drug use rather than recent usage. DETECTION WINDOW ## What is the detection window for a 5-panel hair follicle drug test? The detection window for this test is typically up to 90 days, which corresponds to the length of hair collected. Hair grows about half an inch per month, so a 1.5-inch sample captures drug use over three months. Drugs are deposited in the hair follicle from the bloodstream as hair grows, making this test effective at detecting past drug exposure rather than recent or immediate use. Because the test uses hair rather than [urine](https://amhnationwide.com/urine-drug-testing/), it cannot detect drug use within the last few days. This longer window provides a historical overview of drug use patterns. ## Ready to Learn More About 5-Panel Hair Follicle Drug Testing? Call (877) 731-6362 to discuss the testing process and arrange sample collection with AMH Nationwide. [Call (877) 731-6362](tel:+18777316362)[Contact Us](https://amhnationwide.com/contact-us/) MRO REVIEW ## What role does the Medical Review Officer play in the testing process? Every non-negative test result from the lab is reviewed by a certified Medical Review Officer (MRO). The MRO evaluates the laboratory findings alongside any relevant medical history or prescription information provided by the individual. The MRO confirms whether the positive result is medically justified or if there could be an alternative explanation, such as prescribed medications. This review is a standard part of the 5-panel hair follicle drug test how it works to ensure accuracy and fairness before results are reported to the requester. ORDERING AND COLLECTION ## How do you initiate a 5-panel hair follicle drug test with AMH Nationwide? AMH Nationwide provides the lab order and directs the requester to an appropriate collection site within the five national lab networks. There is no online test ordering available. To initiate testing, contact AMH Nationwide by calling (877) 731-6362. The staff will assist with obtaining the lab order and locating a nearby collection facility. The collection site performs the sample collection under chain of custody protocols and sends the sample to the lab for processing as described. Results, including MRO-reviewed reports of positive or negative status, are then returned to the requester. AMH Reviewed & Maintained By AMH Nationwide Customer Service AMH Nationwide is BBB A+ accredited and routes every order through Quest Diagnostics, LabCorp, Omega Labs, United States Drug Testing Labs, or ExperTox, all of which use multiple lab certification agencies and standards such as [SAMHSA](https://www.samhsa.gov/workplace/drug-testing)-aligned laboratory protocols, the ANSI National Accreditation Board for forensic testing and calibration, the College of American Pathologists (CAP), and Clinical Laboratory Improvement Amendments (CLIA). Every non-negative screen is reviewed by a certified Medical Review Officer as part of standard processing before a result is released, and chain-of-custody documentation is maintained on every collection. This page does not constitute medical or legal advice. **Last updated:** July 2026 You May Also Need [Nail Drug TestingNo head or body hair available? A 5-panel fingernail test covers a similar look-back window.Learn more →](https://amhnationwide.com/5-panel-fingernail-drug-test/)[ChildGuard: Environmental ExposureConcerned about a child or loved one? ChildGuard screens for exposure to drugs in their environment.Learn more →](https://amhnationwide.com/5-panel-hair-drug-test-environmental-exposure-childguard/)[Shorten the Detection WindowShortened options like 30 and 60 day hair panels are also available, useful when a urine test’s 1-3 day window is too short.Learn more →](https://amhnationwide.com/30-day-hair-drug-test/)[Hair ETG Alcohol TestAlso need alcohol testing? The hair ETG test screens for alcohol use, and can be added to the same collection.Learn more →](https://amhnationwide.com/etg-and-peth/) Frequently Asked Questions ## Common Questions Results typically take several days to a week after sample collection, depending on the lab’s workload and confirmation testing requirements. Certain hair treatments like bleaching or dyeing may reduce drug metabolite concentration, but labs account for this during analysis to maintain test accuracy. No, DOT-regulated safety-sensitive testing requires federally certified urine testing under [49 CFR Part 40](https://www.transportation.gov/odapc/part40). Hair testing is used for non-DOT roles. A minimum of 1.5 inches of hair is needed to cover approximately 90 days of drug history for accurate testing. Prescription medications containing tested substances may be identified, but the Medical Review Officer reviews such cases to verify legitimate medical use. No, hair follicle tests reflect drug use over about 90 days and cannot reliably detect very recent drug use within days. Sources • [SAMHSA – Mandatory Guidelines for Federal Workplace Drug Testing Programs](https://www.samhsa.gov/workplace/drug-testing) • [U.S. DOT – 49 CFR Part 40 – Procedures for Transportation Workplace Drug and Alcohol Testing Programs](https://www.transportation.gov/odapc/part40) • [CDC – National Center for Health Statistics – Drug Use FastStats](https://www.cdc.gov/nchs/fastats/drug-use.htm) • [Quest Diagnostics – Employer Solutions, Hair Testing Methodology](https://www.questdiagnostics.com/) • [LabCorp – Occupational Testing Services](https://www.labcorp.com/) ## Ready to get started? AMH Nationwide provides the lab order and arranges collection nationwide. Every non-negative result reviewed by a certified Medical Review Officer. Call (877) 731-6362 or contact us. [Call (877) 731-6362](tel:+18777316362)[Contact Us](https://amhnationwide.com/contact-us/) --- **Need a drug, alcohol, HIV or STD test?** AMH Nationwide is a national lab testing agency operating since 2004, with more than 20,000 collection sites across the United States. Hair, urine, fingernail and blood testing, panels from 5 through 19, for individuals and employers. - Call: (877) 731-6377 - Find a collection site: https://amhnationwide.com/locations/ - Full page: https://amhnationwide.com/5-panel-hair-follicle-drug-test-how-it-works/